Analysis of DNA Microarrays by Non-Destructive Fluorescent Staining Using SYBR® Green II
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چکیده
منابع مشابه
Analysis of DNA microarrays by non-destructive fluorescent staining using SYBR green II.
A simple, non-destructive procedure is described to determine the quality of DNA arrays before they are used. It consists of a preliminary staining step of the DNA microarray by using SYBR green II, a fluorophore with specific affinity for ssDNA, followed by a laser scan analysis. The surface quality, integrity and homogeneity of each DNA spot of the array can thus be assessed. After this preli...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2000
ISSN: 0736-6205,1940-9818
DOI: 10.2144/00291st01